AIIMS CRE, SNO-2024
Medical & Surgical Nursing
Easy
Platelet-rich plasma (PRP) is obtained by centrifuging blood at:
Appeared in: AIIMS CRE, SNO-2024
Explanation
- The preparation of Platelet-Rich Plasma (PRP) requires a 'soft spin' or slow centrifugation to separate heavier cells from the plasma.
- The goal of this initial spin is to pellet the red blood cells and white blood cells while keeping the lighter platelets suspended in the plasma.
- A centrifugation setting of 500g for 10 minutes is a standard parameter for a 'soft spin' that accomplishes this separation effectively.
- This process yields plasma that is rich in platelets, which is the definition of PRP.
Why Other Options Were Wrong
- Option B: A force of 1600g is significantly higher than a typical 'soft spin'. It risks prematurely pelleting the platelets along with the red blood cells, reducing the yield of platelets in the plasma.
- Option C: This represents a 'hard spin'. This high force is used in the second step of a two-spin protocol to concentrate platelets into a pellet from the PRP, or to create Platelet-Poor Plasma (PPP). It is not used for the initial creation of PRP.
- Option D: This force and duration are too low and too short. It would not be sufficient to create the gravitational force needed to effectively separate the dense red blood cells from the plasma, resulting in a poorly separated sample.
Related Visual
- Visual 1: Diagram - A diagram showing two test tubes of centrifuged blood. Label one 'Soft Spin (for PRP)' showing a large layer of plasma with suspended platelets above a pellet of red blood cells. Label the other 'Hard Spin (for PPP)' showing a clear plasma layer above a pellet containing both red blood cells and platelets.
Clinical Relevance
- Nursing practice connection: Knowing Centrifugation parameters for Platelet-Rich Plasma (PRP) preparation helps nurses interpret findings accurately and avoid errors in routine assessment, medication administration, and patient teaching.
- PRP is used therapeutically in orthopedics, dermatology, and wound care to promote healing due to the high concentration of growth factors in platelets.
- Nurses may be involved in patient education about PRP procedures, assisting in the collection of the blood sample, and post-procedure care.
- Understanding the preparation process is crucial for quality control. If a sample is centrifuged incorrectly (e.g., a hard spin instead of a soft spin), the resulting plasma will not be rich in platelets and the therapy will be ineffective.
How to Approach the Question
- First, identify the key term in the question: 'Platelet-rich plasma (PRP)'.
- Recall the basic principle of its preparation: separating heavier red blood cells from lighter platelets, which should remain in the plasma.
- This requires a gentle or 'soft spin'.
- Evaluate the options to find the one that represents a 'soft spin'. Eliminate options that are too aggressive ('hard spin') or too weak (insufficient separation).
- 3000g is a very high force, characteristic of a 'hard spin' used for making platelet-POOR plasma. Eliminate this.
- 100g for 1 minute is very low and short, likely ineffective. Eliminate this.
Concept Tested & Keywords
- Concept Tested: Centrifugation parameters for Platelet-Rich Plasma (PRP) preparation
- Stem keywords: Platelet-rich plasma, PRP, centrifuging blood
- Lead-in keywords: obtained by
Question ID
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