DSSSB - 29 August 2019 (Shift-1)
Applied Microbiology & Infection control
Easy

An example of moist heat is?

Appeared in: DSSSB - 29 August 2019 (Shift-1)

Explanation

  • Moist heat sterilization uses water vapor or steam to kill microorganisms.
  • Steam under pressure, as used in an autoclave, is a classic and highly effective method of moist heat sterilization.
  • The high pressure allows the steam to reach temperatures above the normal boiling point of water (e.g., 121°C at 15 psi), which rapidly denatures and coagulates microbial proteins.
  • This method is efficient because steam has excellent penetrating power, ensuring all surfaces of the items being sterilized are reached.

Why Other Options Were Wrong

  • Option A: Incineration is a method of dry heat sterilization. It involves burning materials at very high temperatures, reducing them to ash. It does not use water or steam.
  • Option C: A hot air oven is a form of dry heat sterilization. It uses heated dry air to kill microorganisms through oxidation. It lacks the moisture component.
  • Option D: Flaming is a dry heat method used for rapid sterilization of small instruments, like inoculation loops in a microbiology lab. It involves passing the object directly through a flame.

Related Visual

Visual explanation — Related Visual
Clinical Relevance
  • Nursing practice connection: This is primarily an exam-oriented knowledge point with limited direct bedside application, so retain Sterilization Methods (Moist vs. Dry Heat) as background academic context rather than a clinical decision trigger.
  • Understanding the difference between moist and dry heat sterilization is critical for nurses to ensure patient safety by preventing healthcare-associated infections (HAIs).
  • Nurses are responsible for ensuring that medical equipment, especially critical items that enter sterile tissue (e.g., surgical instruments, urinary catheters), is properly sterilized before use.
  • Improper sterilization can lead to surgical site infections, sepsis, and other serious complications, increasing patient morbidity, mortality, and healthcare costs.
How to Approach the Question
  • First, identify the key concept in the question, which is "moist heat".
  • Recall the definition of moist heat sterilization: a process that uses steam or hot water to kill microbes.
  • Analyze each option to determine if it fits the definition of moist heat.
  • Option A (Incineration), Option C (Hot air oven), and Option D (Flaming) all use high temperatures without water, classifying them as dry heat methods.
  • Option B (Steam under pressure) explicitly involves steam, which is the hallmark of moist heat sterilization.
  • Therefore, conclude that Steam under pressure (Autoclave) is the correct example of moist heat.
Concept Tested & Keywords
  • Concept Tested: Sterilization Methods (Moist vs. Dry Heat)
  • Stem keywords: moist heat, sterilization
  • Lead-in keywords: example of

Question ID

QRZbu78tqr_VW60Fwfx5a8

Reference Book

E6 Parks TextBook of Preventive & Social Medicine part 1 — Subpart A (pp 26-201 of 604) pp. 126-128, 127-129

E6 Nursing Fundamentals Potter Perry 12e Part 2 p. 231-233

Practise the full DSSSB - 29 August 2019 (Shift-1)

Attempt every question from this paper in a timed mock, then review the full solution for each one.

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